Ewing's sarcoma arising in prostate gland: A case report.

An 18-year-old Thai male presented with weight loss and urinary retention. Physical examination revealed an enlarged prostate gland. Serum prostate specific antigen (PSA) level was 0.3ng/ml. Histologic section demonstrated a small round cell tumor with intracytoplasmic glycogen. Immunohistochemical and ultrastructural studies were compatible with Ewing's sarcoma. Since no other osseous or extraosseous lesions of the same tumor were found in this patient, we concluded that this was a case of primary extraskeletal Ewing's sarcoma of the prostate gland. Case follow-up after one year revealed a good response to chemotherapy.

Pathological study of pigmented dermatofibrosarcoma protuberans.

In this paper we performed morphological studies of Bednar tumor, which is a rare pigmented variant of dermatofibrosarcoma protuberans (DFSP) for evidence of histogenetic origin. Presence of melanosome-containing cells is the only histologic criterion of Bednar tumor, which is different from the ordinary DFSP. Immunohistochemical and ultrastructural examinations revealed that the melanosome-containing cells gave positive reactivity to antibodies to vimentin and S-100 protein, but negative to HMB-45, CD38, Factor VIII, NSE, and KP-1. The melanosomes are in Stage II, III, and IV, suggesting that the pigment was produced within the cell rather than phagocytosed from other cells. The rest of the tumor had similar pattern of immunoreactivity to DFSP such as positivity for CD34 and vimentin, indicating that Bednar tumor and DFSP originated from the same cell line. The ultrastructural feature of non-pigmented cells was similar to perineural cells in the first group and similar to fibroblasts in the second group. DNA flow cytometry in our cases revealed diploid cells with low to intermediate S-phase fraction and no aneuploid cells which were reported in a previous study.

Ultrastructural features of sidero - apoptotic hepatocytes in thalassemia.

Liver tissues from 20 patients with thalassemia were investigated by light and electron microscopy, with focus on ultrastructure of sidero-apoptosis. The results of light microscopy were compared. Sidero-apoptosis was found in 82% in b thal/Hb E disease, 67% in thalassemia Hb H, 100% in and b-thal major, respectively. Regardless of the type of hemoglobin, sidero-apoptosis was present in various degrees. The main criteria used for identification of sidero-apoptosis were marked condensation of chromatin against the nuclear membrane, and fragmentation into nuclear fragments of varying sizes and structures into membrane-bounded sidero-apop-totic bodies. These bodies may be phagocytosed by macrophages or neighboring cells or remain free. However, the cell may also shrink into a dense, rounded mass, as a single sidero-apoptotic body. Sidero-apoptoic bodies were similar to acidophilic bodies, or Councilman bodies, commonly found in viral hepatitis. This preliminary report suggestsd that sidero-apoptosis is a features of human liver pathology in thalassemia and may be due to hepatotoxicity of iron overload. In addition, many factors or other agents-hormone, killer cells, cytokines, various physical, chemical and viral agents should be considered. We propose the term "sidero-apoptosis" to describe the features resenbling acidophilic bodies commonly seen in viral hepatitis but with a heavy depositi of iron.

Primary ciliary dyskinesia: A case presentation and A current review.

We present a 14 year-old girl with a history of chronic rhinorrhoea and nasal obstruction. Physical findings showed huge nasal polyp with broadening of the nasal bridge, perforation of the left ear drum and a right middle ear effusion. Despite a strict program of nasal saline toilet, broad-spectrum antibiotics covering beta-lactamase producing organisms, and meticulous technique of endoscopic sinus surgery, the patient's symptoms and nasal polyp reappeared very quickly. A set of investigations had been done in order to confirm and rule out suspected diseases. The electron microscopic study of the patient's nasal mucosa revealed partial absence of inner dynein arms and extra singlet at the center in some axoneme compatible with Primary ciliary dyskinesia.

Pulmonary leiomyoma in an HIV-infected child: A case report.

Smooth muscle tumors, benign or malignant, are rare in childhood. However, a higher incidence is found in HIV infected children with a reported association with Epstein-Barr Virus. We report a case of a 6-year-old HIV-infected girl who presented with a right upper lung mass and pulmonary tuberculosis. Histopathology of the resected mass showed a bronchiolar leiomyoma with adjacent noncaseous granulomas. Staining for AFB was negative. Polymerase chain reaction amplification for Mycobacterium tuberculosis complex was weakly positive. PCR amplification for EBV yielded a negative result.

Mixed carcinoid – adenocarcinoma of vermifrom appendix: A case report.

Three histology types of appendiceal carcinoid are classified as: tubular carcinoid or classic carcinoid; goblet cell carcinoid or adenocarcinoid; and mixed carcinoid-adenocarcinoma. Each have a different prognosis and method of treatment. We report a 56-year-old Thai woman who presented with an intermittent abdominal pain in the right lower quadrant for nine months and was finally diagnosed as acute appendicitis. Appendicectomy was performed and the patients was unexpectedly found to have a mixed carcinoid-adenocarcinoma. Subsequently, hemicolectomy was carry out to provide appropriate treatment. Immunostains and ultratructural studies were performed to confirm the combination of endocrine and glandular differentiation.